The Joint Cytometry Unit, formed by the Josep Carreras Leukemia Research Institute and the company Cytognos S.L., has developed a new commercial reagent that helps determine the prognosis of patients with Acute Myeloid Leukemia at the time of diagnosis.
Acute Myeloid Leukemia (AML) is a very aggressive type of hematological cancer caused by the abnormal proliferation of myeloid stem cells (myeloblasts). AML initially manifests itself in the bone marrow but can rapidly spread to the blood and sometimes affects other parts of the body. It is the most common hematologic cancer in adults and is associated with a low probability of recovery and a relatively high early mortality rate.
Investigating AML is a complex task, due to the clonal heterogeneity of the pathological population (there is great variation both among the malignant cells of the same patient and among different patients). Therefore, any advance that allows prediction of disease progression is of paramount importance to offer a better and personalized response to the treatment plan of each individual.
The Functional Cytomics Group of the Josep Carreras Leukemia Research Institute, led by Dr. Jordi Petriz, has shown that higher cellular alkaline phosphatase activity in leukemic myeloid precursors is significantly associated with a higher risk of relapse or resistance to treatment and mortality (Rico, et al. 2019).
Based on this research and under license from the Josep Carreras Institute, Cytognos S.L. has developed a commercial kit, which is available under the name APLS-AML (Alkaline Phosphatase Live Stain-Acute Myeloid Leukemia). The kit uses a 4-colour antibody combination and should be used together with the profluorescent substrate of the alkaline phosphatase enzyme.
The kit is based on methodologies developed at the Josep Carreras Institute that allow the minimum manipulation of the sample, combining functional analysis and cellular immunophenotyping to evaluate the enzymatic activity in leukemic cells.
The Josep Carreras Institute and Cytognos S.L. are both convinced that the joint efforts of the two institutions, which are already bearing fruit, will boost technology and knowledge about hematological cancers, contributing significantly to the diagnosis and treatment of these diseases.